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MB Sample ID: SA086927

Local Sample ID:S00031821
Subject ID:SU001293
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU001293
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
S00031821SA086927FL012879Day 90Time Point

Collection:

Collection ID:CO001287
Collection Summary:Not available.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR001308
Treatment Summary:Not available.

Sample Preparation:

Sampleprep ID:SP001301
Sampleprep Summary:Two-step solvent extraction. Supernatants are combined, dried, and re-suspended for LCMS separation by RPLC and measurements by ESI- QQQ MRM methods. Analytes are reported as uM, and CV's are generally <15%.
Sampleprep Protocol Filename:Bile_acids_analysis.doc

Combined analysis:

Analysis ID AN002039
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260
Column Waters Acquity BEH C18 (50 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH001479
Methods Filename:2014-07-30-Bile_acid-full-BEH-C18-I.m.zip
Instrument Name:Agilent 1260
Column Name:Waters Acquity BEH C18 (50 x 2mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001891
Analysis ID:AN002039
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NA
Ion Mode:POSITIVE
Acquisition Parameters File:2014-07-30-Bile_acid-full-BEH-C18-I.m.zip
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