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MB Sample ID: SA093950
| Local Sample ID: | S00030116 |
| Subject ID: | SU001366 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001366 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S00030116 | SA093950 | FL013445 | Treat | ACE/ARB Inhibitor |
| S00030116 | SA093950 | FL013445 | db/db diabetic | control/diabetic |
| S00030116 | SA093950 | FL013445 | eNOS -/- | eNOS background |
Collection:
| Collection ID: | CO001361 |
| Collection Summary: | Kidney samples snap-frozen and stored at -80 until use. |
| Sample Type: | Kidney |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001381 |
| Treatment Summary: | Treatment group mice received 20 mg/kg/d lisinopril + 30 mg/kg/d losartan) for 12 wk (starting at 12 wk age), controls didn't receive any drugs. |
Sample Preparation:
| Sampleprep ID: | SP001374 |
| Sampleprep Summary: | Standard tissue lipidomics protocol |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN002149 | AN002150 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001572 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001573 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002000 |
| Analysis ID: | AN002149 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids |
| Ion Mode: | POSITIVE |
| MS ID: | MS002001 |
| Analysis ID: | AN002150 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids |
| Ion Mode: | NEGATIVE |
