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MB Sample ID: SA093957
Local Sample ID: | S00030127 |
Subject ID: | SU001366 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001366 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S00030127 | SA093957 | FL013446 | Treat | ACE/ARB Inhibitor |
S00030127 | SA093957 | FL013446 | db/m control | control/diabetic |
S00030127 | SA093957 | FL013446 | eNOS +/+ | eNOS background |
Collection:
Collection ID: | CO001361 |
Collection Summary: | Kidney samples snap-frozen and stored at -80 until use. |
Sample Type: | Kidney |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001381 |
Treatment Summary: | Treatment group mice received 20 mg/kg/d lisinopril + 30 mg/kg/d losartan) for 12 wk (starting at 12 wk age), controls didn't receive any drugs. |
Sample Preparation:
Sampleprep ID: | SP001374 |
Sampleprep Summary: | Standard tissue lipidomics protocol |
Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
Analysis ID | AN002149 | AN002150 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
Column | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH001572 |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001573 |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002000 |
Analysis ID: | AN002149 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids |
Ion Mode: | POSITIVE |
MS ID: | MS002001 |
Analysis ID: | AN002150 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids |
Ion Mode: | NEGATIVE |