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MB Sample ID: SA113582
Local Sample ID: | S00034905 |
Subject ID: | SU001472 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001472 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S00034905 | SA113582 | FL014337 | 6 hr | Hours of Exposure |
S00034905 | SA113582 | FL014337 | 20 uM DCVC | Treatment |
Collection:
Collection ID: | CO001467 |
Collection Summary: | Standard tissue culture sample collection method |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR001487 |
Treatment Summary: | Exposure to cell culture media (control) or 20 µM S-(1,2-dichlorovinyl)-L-cysteine (DCVC) for 6 or 12 hours |
Sample Preparation:
Sampleprep ID: | SP001480 |
Sampleprep Summary: | Standard TCS-plus assay sample preparation protocol |
Sampleprep Protocol Filename: | SP_A043_TCA_Plus_20170713.PDF |
Combined analysis:
Analysis ID | AN002337 | AN002338 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1200 | Agilent 1200 |
Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF |
Ion Mode | NEGATIVE | NEGATIVE |
Units | area/ug protein | pmol/ug |
Chromatography:
Chromatography ID: | CH001713 |
Chromatography Summary: | HILIC NH2 chromatography |
Instrument Name: | Agilent 1200 |
Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
Solvent A: | 100% water; 5mM ammonium acetate, pH 9.9, adjust with ammonium hydroxide |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002179 |
Analysis ID: | AN002337 |
Instrument Name: | Agilent 6520 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Quantitative data analysis using Agilent Quant software |
Ion Mode: | NEGATIVE |
MS ID: | MS002180 |
Analysis ID: | AN002338 |
Instrument Name: | Agilent 6520 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Semi-quantitative data analysis using Agilent Quant software |
Ion Mode: | NEGATIVE |