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MB Sample ID: SA113587
| Local Sample ID: | S00034899 |
| Subject ID: | SU001472 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001472 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S00034899 | SA113587 | FL014338 | 6 hr | Hours of Exposure |
| S00034899 | SA113587 | FL014338 | No Treatment | Treatment |
Collection:
| Collection ID: | CO001467 |
| Collection Summary: | Standard tissue culture sample collection method |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001487 |
| Treatment Summary: | Exposure to cell culture media (control) or 20 µM S-(1,2-dichlorovinyl)-L-cysteine (DCVC) for 6 or 12 hours |
Sample Preparation:
| Sampleprep ID: | SP001480 |
| Sampleprep Summary: | Standard TCS-plus assay sample preparation protocol |
| Sampleprep Protocol Filename: | SP_A043_TCA_Plus_20170713.PDF |
Combined analysis:
| Analysis ID | AN002337 | AN002338 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1200 | Agilent 1200 |
| Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | area/ug protein | pmol/ug |
Chromatography:
| Chromatography ID: | CH001713 |
| Chromatography Summary: | HILIC NH2 chromatography |
| Instrument Name: | Agilent 1200 |
| Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
| Solvent A: | 100% water; 5mM ammonium acetate, pH 9.9, adjust with ammonium hydroxide |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002179 |
| Analysis ID: | AN002337 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Quantitative data analysis using Agilent Quant software |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002180 |
| Analysis ID: | AN002338 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Semi-quantitative data analysis using Agilent Quant software |
| Ion Mode: | NEGATIVE |
