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MB Sample ID: SA124130
| Local Sample ID: | 10_B1-0_Liver_syngenic_7days_170427_UKy_GCH_rep1-polar-ICMS_A |
| Subject ID: | SU001527 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001527 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 10_B1-0_Liver_syngenic_7days_170427_UKy_GCH_rep1-polar-ICMS_A | SA124130 | FL015007 | syngenic | Treatment Protocol |
Collection:
| Collection ID: | CO001522 |
| Collection Summary: | Mouse is sacrificed and tissues are harvested. |
| Collection Protocol ID: | mouse_tissue_collection |
| Sample Type: | Multiple tissues |
Treatment:
| Treatment ID: | TR001542 |
| Treatment Summary: | Mouse with allogenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with no treatment. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with syngenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. |
| Treatment Protocol ID: | allogenic naive syngenic |
Sample Preparation:
| Sampleprep ID: | SP001535 |
| Sampleprep Summary: | Tissue is frozen in liquid nitrogen to stop metabolic processes. Polar extraction from homogenate, lypholized, and frozen. Protein extraction and quantification. Before going into the IC-FTMS the frozen sample is reconstituted in water. Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. |
| Sampleprep Protocol ID: | frozen_tissue_grind; IC-FTMS_preparation; protein_extraction; polar_extraction; tissue_quench |
| Sampleprep Protocol Filename: | 4D_17Jun4_Fan_Prot_Quant.pdf 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf |
Combined analysis:
| Analysis ID |
|---|
| Analysis type |
| Chromatography type |
| Chromatography system |
| Column |
| MS Type |
| MS instrument type |
| MS instrument name |
| Ion Mode |
| Units |
Chromatography:
| Chromatography ID: | CH001785 |
| Chromatography Summary: | Targeted IC |
| Instrument Name: | Thermo Dionex ICS-5000+ |
| Column Name: | Dionex IonPac AS11-HC (250 x 2mm,4um) |
| Chromatography Type: | Ion exchange |
MS:
