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MB Sample ID: SA124324
| Local Sample ID: | 12_B1-2_Spleen_syngenic_7days_170427_UKy_GCH_rep3-polar-NMR_A |
| Subject ID: | SU001540 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001540 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 12_B1-2_Spleen_syngenic_7days_170427_UKy_GCH_rep3-polar-NMR_A | SA124324 | FL015046 | syngenic | Treatment Protocol |
Collection:
| Collection ID: | CO001535 |
| Collection Summary: | Mouse is sacrificed and tissues are harvested. |
| Collection Protocol ID: | mouse_tissue_collection |
| Sample Type: | Multiple tissues |
Treatment:
| Treatment ID: | TR001555 |
| Treatment Summary: | Mouse with allogenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with no treatment. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with syngenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. |
| Treatment Protocol ID: | allogenic naive syngenic |
Sample Preparation:
| Sampleprep ID: | SP001548 |
| Sampleprep Summary: | Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. Polar extraction from homogenate, lypholized, and frozen. Tissue is frozen in liquid nitrogen to stop metabolic processes. Protein extraction and quantification. |
| Sampleprep Protocol ID: | polar_extraction; frozen_tissue_grind; tissue_quench; protein_extraction |
| Sampleprep Protocol Filename: | 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf 4D_17Jun4_Fan_Prot_Quant.pdf |
Analysis:
| MB Sample ID: | SA124324 |
| Analysis ID: | AN002441 |
| Analysis Type: | NMR |
| Num Factors: | 3 |
| Num Metabolites: | 47 |
| Units: | normalized peak area |
NMR:
| NMR ID: | NM000180 |
| Analysis ID: | AN002441 |
| Instrument Name: | Agilent 600 |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | HC-HSQC |
| Standard Concentration: | 0.5 mM |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | cryogenic triple resonance HCN |
| NMR Solvent: | D20 |
| NMR Tube Size: | 1.7 mm |
| Shimming Method: | gradient shimming |
| Pulse Sequence: | gHSQCAD |
| Water Suppression: | presaturation |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 15 celsius |
| Acquisition Time: | 2 s |
| Relaxation Delay: | 4 s |
| Baseline Correction Method: | Bernstein Polynomial |
