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MB Sample ID: SA124533
| Local Sample ID: | 11_B1-1_SI_syngenic_7days_170427_UKy_GCH_rep2-lipid |
| Subject ID: | SU001545 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001545 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 11_B1-1_SI_syngenic_7days_170427_UKy_GCH_rep2-lipid | SA124533 | FL015070 | syngenic | Treatment Protocol |
Collection:
| Collection ID: | CO001540 |
| Collection Summary: | Mouse is sacrificed and tissues are harvested. |
| Collection Protocol ID: | mouse_tissue_collection |
| Sample Type: | Multiple tissues |
Treatment:
| Treatment ID: | TR001560 |
| Treatment Summary: | Mouse with allogenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with no treatment. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with syngenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. |
| Treatment Protocol ID: | allogenic naive syngenic |
Sample Preparation:
| Sampleprep ID: | SP001553 |
| Sampleprep Summary: | Lipid extraction from homogenate. Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. Protein extraction and quantification. Tissue is frozen in liquid nitrogen to stop metabolic processes. |
| Sampleprep Protocol ID: | frozen_tissue_grind; protein_extraction; tissue_quench; lipid_extraction |
| Sampleprep Protocol Filename: | 4D_17Jun4_Fan_Prot_Quant.pdf 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf |
Combined analysis:
| Analysis ID | AN002447 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | None |
| Column | None |
| MS Type | ESI |
| MS instrument type | FTMS |
| MS instrument name | Orbitrap Fusion |
| Ion Mode | POSITIVE |
| Units | peak_height |
Chromatography:
| Chromatography ID: | CH001792 |
| Chromatography Summary: | None |
| Instrument Name: | None |
| Column Name: | None |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS002270 |
| Analysis ID: | AN002447 |
| Instrument Name: | Orbitrap Fusion |
| Instrument Type: | FTMS |
| MS Type: | ESI |
| MS Comments: | Measurements made using direct infusion (nano-electrospray) FTMS. |
| Ion Mode: | POSITIVE |
