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MB Sample ID: SA124566
Local Sample ID: | 10_B1-0_Spleen_syngenic_7days70427_UKy_GCH_rep1-lipid |
Subject ID: | SU001547 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001547 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
10_B1-0_Spleen_syngenic_7days70427_UKy_GCH_rep1-lipid | SA124566 | FL015076 | syngenic | Treatment Protocol |
Collection:
Collection ID: | CO001542 |
Collection Summary: | Mouse is sacrificed and tissues are harvested. |
Collection Protocol ID: | mouse_tissue_collection |
Sample Type: | Multiple tissues |
Treatment:
Treatment ID: | TR001562 |
Treatment Summary: | Mouse with allogenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with no treatment. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with syngenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. |
Treatment Protocol ID: | allogenic naive syngenic |
Sample Preparation:
Sampleprep ID: | SP001555 |
Sampleprep Summary: | Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. Lipid extraction from homogenate. Protein extraction and quantification. Tissue is frozen in liquid nitrogen to stop metabolic processes. |
Sampleprep Protocol ID: | lipid_extraction; frozen_tissue_grind; tissue_quench; protein_extraction |
Sampleprep Protocol Filename: | 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf 4D7Jun4_Fan_Prot_Quant.pdf |
Combined analysis:
Analysis ID | AN002449 |
---|---|
Analysis type | MS |
Chromatography type | None (Direct infusion) |
Chromatography system | None |
Column | None |
MS Type | ESI |
MS instrument type | FTMS |
MS instrument name | Orbitrap Fusion |
Ion Mode | POSITIVE |
Units | peak_height |
Chromatography:
Chromatography ID: | CH001794 |
Chromatography Summary: | None |
Instrument Name: | None |
Column Name: | None |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS002272 |
Analysis ID: | AN002449 |
Instrument Name: | Orbitrap Fusion |
Instrument Type: | FTMS |
MS Type: | ESI |
MS Comments: | Measurements made using direct infusion (nano-electrospray) FTMS. |
Ion Mode: | POSITIVE |