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MB Sample ID: SA126088
| Local Sample ID: | SIHUMI_Tau_4_B |
| Subject ID: | SU001570 |
| Subject Type: | Bacteria |
| Subject Species: | SIHUMI consortia |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001570 |
| Subject Type: | Bacteria |
| Subject Species: | SIHUMI consortia |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SIHUMI_Tau_4_B | SA126088 | FL015484 | 3 h | Incubation_time |
| SIHUMI_Tau_4_B | SA126088 | FL015484 | No | Sulfoquinovose (4 mM) |
| SIHUMI_Tau_4_B | SA126088 | FL015484 | Yes | Taurine (20 mM) |
| SIHUMI_Tau_4_B | SA126088 | FL015484 | Yes | Incubated with SIHUMI consortia |
| SIHUMI_Tau_4_B | SA126088 | FL015484 | no | Incubated with Bilophila wadsworthia |
Collection:
| Collection ID: | CO001565 |
| Collection Summary: | For each 10 ml incubation, DS medium supplemented with either taurine (20 mM) or sulfoquinovose (SQ, 4 mM) was inoculated with the SIHUMI bacteria or the SIHUMI consortium and B. wadsworthia. Control incubations of medium containing only sulfonates or bacteria were included. The incubations were conducted under anoxic conditions at 37 °C in duplicates. Samples (600 µl) were withdrawn after 0, 3, 24 and 48 h for quantification of sulfoquinovose (SQ), 2,3-dihydroxypropane-1-sulfonate (DHPS), taurine and isethionate. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR001585 |
| Treatment Summary: | For each 10 ml incubation, DS medium supplemented with either taurine (20 mM) or sulfoquinovose (SQ, 4 mM) was inoculated with the SIHUMI bacteria or the SIHUMI consortium and B. wadsworthia. Control incubations of medium containing only sulfonates or bacteria were included. The incubations were conducted under anoxic conditions at 37 °C in duplicates. Samples (600 µl) were withdrawn after 0, 3, 24 and 48 h for quantification of sulfoquinovose (SQ), 2,3-dihydroxypropane-1-sulfonate (DHPS), taurine and isethionate. |
Sample Preparation:
| Sampleprep ID: | SP001578 |
| Sampleprep Summary: | For the analysis of sulfonates by LC-MS/MS-MRM, aliquots of 250 µl for each sample were centrifuged (14 000 x g, 4 °C, 5 min) and 50 µl of the supernatant stored at – 20 °C until further processing. Samples were thawed, centrifuged at 18 000 x g at RT for 2 min and the supernatant was diluted 1:10 000 in 50% aqueous acetonitrile. Subsequently, 500 µl of each sample were placed in glass vials (Wicom, Heppenheim, Germany) and stored at - 80 °C until LC-MS/MS analysis. |
| Sampleprep Protocol Filename: | sven_haange_Sample_preparation-application_experiment.pdf |
Combined analysis:
| Analysis ID | AN002479 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Dionex 3000 HPLC (Dionex,Sunnyvale, USA) |
| Column | BEH amide (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | mM |
Chromatography:
| Chromatography ID: | CH001816 |
| Methods Filename: | sven_haange_20201002_055740_MS-parameters.pdf sven_haange_20201002_055740_LC_parameters-sulfonates.pdf |
| Chromatography Comments: | BEH amide column, 2.1 x 100 mm, 1.7 µm, Waters, Milford, USA |
| Instrument Name: | Dionex 3000 HPLC (Dionex,Sunnyvale, USA) |
| Column Name: | BEH amide (100 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002299 |
| Analysis ID: | AN002479 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | As software used Analyst 1.6.2 (Sciex) |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | sven_haange_20201002_055740_LC_parameters-sulfonates.pdf |
