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MB Sample ID: SA137763
| Local Sample ID: | U_488 |
| Subject ID: | SU001696 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40-75 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001696 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40-75 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| U_488 | SA137763 | FL017538 | Opium User | Phenotype |
Collection:
| Collection ID: | CO001689 |
| Collection Summary: | Non-fasted urine samples |
| Sample Type: | Urine |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001709 |
| Treatment Summary: | NA |
Sample Preparation:
| Sampleprep ID: | SP001702 |
| Sampleprep Summary: | Aliquots of each de-identified sample were shipped to the NIH ERCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 298 study urine samples were thawed at 4°C overnight. Aliquots of 400 µL of study samples were transferred to 2.0mL pre-labeled LoBind Eppendorf tubes. Aliquots of 400 µL of analytical quality control (QC) external pooled urine samples were also transferred to 2.0mL pre-labeled LoBind Eppendorf tubes. All samples were mixed with 300uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH in D2O. The tubes were vortexed for 5 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | Described in summary |
Analysis:
| MB Sample ID: | SA137763 |
| Analysis ID: | AN002654 |
| Laboratory Name: | ERCMRC |
| Analysis Type: | NMR |
| Acquisition Date: | March 14 to 20, 2018 |
| Software Version: | TopSpin 3.5 |
| Operator Name: | Kevin Knagge |
| Detector Type: | NMR |
| Data Format: | fid, 1r |
| Rt Units: | No RT data |
| Units: | ppm |
NMR:
| NMR ID: | NM000190 |
| Analysis ID: | AN002654 |
| Instrument Name: | Avance III 700 MHz NMR Spectrometer |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | 3015 Hz, D2O |
| Standard Concentration: | 0.5 mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | CP QCI/HPCN |
| NMR Solvent: | 90% H2O, 10% D2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | topshim |
| Pulse Sequence: | noesygppr1d |
| Water Suppression: | pre-saturation |
| Pulse Width: | 14 microseconds |
| Power Level: | 12.3 Watts |
| Receiver Gain: | 36 |
| Offset Frequency: | 3289.61 Hz |
| Presaturation Power Level: | 0.0000964 Watts |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 25 C |
| Number Of Scans: | 32 |
| Dummy Scans: | 4 |
| Acquisition Time: | 3.9 seconds |
| Relaxation Delay: | 2 seconds |
| Spectral Width: | 12 ppm |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 32768 |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | none |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS |
| Binned Increment: | 0.04ppm |
| Binned Data Excluded Range: | 4.69-4.90ppm (water), 5.50-6.00ppm (urea) |
