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MB Sample ID: SA151814
| Local Sample ID: | Peromyscus_22332_LPS_Plasma |
| Subject ID: | SU001731 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus;Peromyscus leucopus |
| Taxonomy ID: | 10090;10041 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001731 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus;Peromyscus leucopus |
| Taxonomy ID: | 10090;10041 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Peromyscus_22332_LPS_Plasma | SA151814 | FL017823 | lipopolysaccharide | Sample Type |
Collection:
| Collection ID: | CO001724 |
| Collection Summary: | At 4.0 hr after LPS injection the animals were euthanized. After opening the chest, exsanguination was performed by cardiac puncture and blood was transferred to a heparin sulfate coated tubes (Becton-Dickinson Microtainer). Anticoagulated blood was centrifuged to pellet blood cells for 3 min at 4600 x g at 4° C. Plasma and blood pellet was kept separately at -80° C until further analysis. |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001744 |
| Treatment Summary: | Animals were anesthetized with isoflurane and injected i.p. with a single dose of E. coli O111:B4 LPS at a concentration of 10 µg/gm body weight in a 50 µl volume as described above. The control group was anesthetized and then injected with the 0.9% saline alone. The experiment started at 0800 h with 10 min intervals between animals and with alteration of LPS and control injections. At 4.0 hr after their injection the animals were euthanized. After opening the chest, exsanguination was performed by cardiac puncture and blood was transferred to a heparin sulfate coated tubes (Becton-Dickinson Microtainer). Anticoagulated blood was centrifuged to pellet blood cells for 3 min at 4600 x g at 4° C. Plasma and blood pellet was kept separately at -80° C until further analysis. |
| Treatment Compound: | E. coli O111:B4 LPS |
| Treatment Route: | i.p. |
| Treatment Dose: | 10 µg/gm body weight |
| Treatment Dosevolume: | 50 µl volume |
| Treatment Vehicle: | sterile, endotoxin-free 0.9% saline |
| Animal Anesthesia: | isoflurane |
Sample Preparation:
| Sampleprep ID: | SP001737 |
| Sampleprep Summary: | Metabolites were extracted from aliquots of 40 µL of plasma with 75% LC-MS grade methanol, supernatants were dried, resuspended in 50% methanol, 10 µL samples were subjected to liquid chromatography and mass spectrometry. Pooled quality control sample was prepared by mixing small aliquot (10 µL) of each plasma sample in the study. |
Combined analysis:
| Analysis ID | AN002701 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1260 |
| Column | Agilent Poroshell 120 EC-C8 (100 x 2.1mm,2.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001993 |
| Instrument Name: | Agilent 1260 |
| Column Name: | Agilent Poroshell 120 EC-C8 (100 x 2.1mm,2.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | non-linear gradient |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002499 |
| Analysis ID: | AN002701 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | An aliquot of sample was injected into LC-MS to acquire data in positive mode. Data acquisition and analysis were achieved using MassHunter software. |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 4000V |
| Dry Gas Temp: | 300 degree C |
| Fragment Voltage: | 120V |
| Gas Pressure: | 45 |
