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MB Sample ID: SA152305
| Local Sample ID: | 6--5 |
| Subject ID: | SU001744 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001744 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 6--5 | SA152305 | FL017911 | High Dose Ricin | Treatment |
| 6--5 | SA152305 | FL017911 | Overnight | Timepoint |
Collection:
| Collection ID: | CO001737 |
| Collection Summary: | Livers were removed from mice immediately following sacrifice and stored at -80 C. Before metabolite extraction, livers were homogenized into powder with mortar and pestle over liquid nitrogen. |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR001757 |
| Treatment Summary: | Mice were injected via intraperitoneal (IP) route with either ricin or saline in 10 microliter per g body weight. Mice in all but the fasted group were given food and water ad libitum. Fasted mice were transferred to new cages with fresh bedding where they had food withheld while given water ad libitum. Mice were sacrificed at 2h (ricin), 8h (ricin and fasted), and overnight (16-22h, control, ricin, fasted). |
Sample Preparation:
| Sampleprep ID: | SP001750 |
| Sampleprep Summary: | Microcentrifuge tubes with 30 mg of frozen liver tissue had 500 uL of ice-cold acetone and three Zirconia beads added to them. Tissues were lysed and homogenized, then sonicated and centrifuged. The supernatant was transferred to a new tube and 500 uL of ice-cold MeOH/H2O was added to the pellet. The pellet was sonicated and centrifuged again. The supernatant was combined with the acetone fraction and vacuum dried. Dried pellets were resuspended in acetonitrile/H2O with 1% Formic Acid, vortexed, sonicated, and centrifuged. 180 uL of eluate was used for LCMS analysis. |
Combined analysis:
| Analysis ID | AN002719 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 |
| Column | Waters Acquity BEH HILIC (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6530 QTOF |
| Ion Mode | POSITIVE |
| Units | Intensity |
Chromatography:
| Chromatography ID: | CH002007 |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters Acquity BEH HILIC (150 x 2.1mm,1.7um) |
| Column Pressure: | 600 bar |
| Column Temperature: | 40 |
| Flow Gradient: | 100% B, 55% B, 20% B, 100%B |
| Flow Rate: | 0.400 mL/min |
| Retention Time: | 25 min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002516 |
| Analysis ID: | AN002719 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | mzML formatting acquired in ProteoWizard msconvert, Feature analysis performed in XCMS Online v3.7.1 |
| Ion Mode: | POSITIVE |
