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MB Sample ID: SA155451
| Local Sample ID: | 471 _2 |
| Subject ID: | SU001762 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001762 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Not applicable |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 471 _2 | SA155451 | FL018325 | 3 months | Sample Code |
| 471 _2 | SA155451 | FL018325 | Q4 | QTL |
Collection:
| Collection ID: | CO001755 |
| Collection Summary: | Blood samples were collected for this study. Plasma was separated within 30 minutes after the blood collection by centrifugation at room temperature. The plasma samples were stored at −80 °C until analyzed. |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001775 |
| Treatment Summary: | Sample were stored at -80 as it is, no treatment done. |
Sample Preparation:
| Sampleprep ID: | SP001768 |
| Sampleprep Summary: | The serum samples were extracted as follows after randomization of the samples. 90 µl of ecetonitrile (containing the BA+PFAS internal standard mixture (c=200 ng/mL PFASs and 1000 ng/mL Bile acids in acetonitrile) was added to the 40 µl of serum samples. The samples were then vortexted and centrifuged (9600 RCF, 10 minutes). 90 µl of the supernatant was collected and evaporated to dryness. The samples were reconstituted with 40 µl of MeOH/H2O (40%/60%). |
Combined analysis:
| Analysis ID | AN002752 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity UPLC |
| Column | BEH C18 |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6210 TOF |
| Ion Mode | NEGATIVE |
| Units | ng/ml |
Chromatography:
| Chromatography ID: | CH002033 |
| Chromatography Summary: | Column name, BEH C18 (2.1 x 100 mm, particle size 1.7 µm) (Waters Corporation, Milford, MA, USA) |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | BEH C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002549 |
| Analysis ID: | AN002752 |
| Instrument Name: | Agilent 6210 TOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | PFASs were analysed on a UHPLC-qTOF/MS (Agilent Technologies, Santa Clara, CA, The United States of America) with Acquity UPLC®, BEH C18 (2.1 x 100 mm, particle size 1.7 µm) (Waters Corporation, Milford, MA, The United States of America) column at 50°C with a C18 pre-column for column protection (Waters Corporation, Wexford, Ireland). Mobile phases used for the sample analysis were A: 2mM NH4Ac in H2O:MeOH (70:30) and B: 2mM NH4Ac in MeOH. NH4Ac was used as ionization agent. The samples were kept at 10°C during the whole sample acquisition and 1 µl of the sample volume was injected. The flow rate was set to 0.4 ml/min and the gradient started with 95%A and 5%B with a change after 1.5 minute to 70%A and 30%B, which followed a change after 4.5 minutes to 30%A and 70%B, the last change was after 7.5 minutes with 100%B until the end of run. Gradient program was 18 minutes long. 13 minutes for sample analysis and 5 minutes for clean-up, including equilibration in the end of the run. Maximum pressure limit in the binary pump was set on 850 bar. Dual jet stream electrospray (dual ESI) ion source was used and the ion polarity was on negative mode. The capillary voltage and the nozzle voltage were kept at 4500 V and 1500 V. The N2 pressure was set on 21 psi, with the sheath gas flow as 11 L/min and temperature at 379°C for the nebulizer. The data was acquired with MassHunter B.06.01 software (Agilent Technologies, Santa Clara, CA, The United States of America). MS data processing was performed using open source software MZmine 2.52 (Pluskal et al. 2010). |
| Ion Mode: | NEGATIVE |
