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MB Sample ID: SA156649
| Local Sample ID: | 1 |
| Subject ID: | SU001767 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001767 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 1 | SA156649 | FL018363 | High-risk T1D | Condition |
Collection:
| Collection ID: | CO001760 |
| Collection Summary: | Blood samples (~20 mL/subject in EDTA) were collected from consented male/female subjects considered at high risk for T1D during routine visits as part of the ongoing TrialNet’s Natural History Study of the Development of Type 1 Diabetes (Pathway to Prevention Study) TN-01 study (n=4). |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR001780 |
| Treatment Summary: | Subjects in the TN-01 study are tested semi-annually for the appearance of new or additional autoantibodies and are evaluated metabolically by oral glucose tolerance test (OGTT) to assess their progression toward clinical diagnosis of T1D. Samples from healthy subjects (n=4) were collected as part of another study approved by the IRB of the University of Miami (study number 11995-115). These trials are conducted in accordance with the principles of the Declaration of Helsinki and consistent with the Good Clinical Practice guidelines of the International Conference on Harmonization. The protocol for the ancillary study, under which the current multi-omics analyses were performed, was approved by TrialNet (study ID number 195) and its IRB. The four high-risk subjects in the present report were staged for their risk level according to the TrialNet staging/scoring system, which considers family history, genetic susceptibility according to haplotype (e.g., HLA-DQ/DR), the number of autoantibodies, and OGTT results as follows: low-risk (1 autoantibody and normal OGTT); moderate-risk (2–3 autoantibodies and normal OGTT); high-risk (4–5 autoantibodies and normal OGTT); and very high-risk (4–5 autoantibodies and abnormal OGTT). |
Sample Preparation:
| Sampleprep ID: | SP001773 |
| Sampleprep Summary: | See uploaded protocol |
| Sampleprep Protocol Filename: | Toadpup1956_20210209_120248_PR_SP_PlasmaPrep_non-targeted_MBX.pdf |
| Sampleprep Protocol Comments: | Please treat these as confidential disclosures of in-house sample prep. |
Combined analysis:
| Analysis ID | AN002760 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975B |
| Ion Mode | POSITIVE |
| Units | minutes |
Chromatography:
| Chromatography ID: | CH002040 |
| Methods Filename: | Toadpup1956_20210209_120248_PR_MS_Data_acquisition_protocol.pdf |
| Instrument Name: | Agilent 6890N |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002557 |
| Analysis ID: | AN002760 |
| Instrument Name: | Agilent 5975B |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | Acquired using Agilent MassHunter Acquisition software. Feature assignment completed using AMDIS Analysis. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Toadpup1956_20210209_120248_PR_MS_Data_acquisition_protocol.pdf |
