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MB Sample ID: SA162494
| Local Sample ID: | GG_8a |
| Subject ID: | SU001803 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Genotype Strain: | HT115 |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | CGC - caenorhabditis genetic center - University of Minesota |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001803 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Genotype Strain: | HT115 |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | CGC - caenorhabditis genetic center - University of Minesota |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| GG_8a | SA162494 | FL019203 | individual batch8 | sample_type |
Collection:
| Collection ID: | CO001796 |
| Collection Summary: | Collection of the two sample types was carried out very differently and further information can be seen in the attached protocols. Both C. elegans and E. coli were grown in bioreactors washed and stored at -80 freezer. |
| Collection Protocol Filename: | goncalog_20210305_191823_PR_CO_BioStat_protocol_ecoli.docx AOS_Extraction_Protocol_09March2020.docx |
| Sample Type: | C. elegans |
Treatment:
| Treatment ID: | TR001816 |
| Treatment Summary: | The groups of samples to be compared are different combinations of multiple samples to reduce variance. Therefore the treatment is just different schema that is described in the puclished manuscript and protocols attached |
Sample Preparation:
| Sampleprep ID: | SP001809 |
| Sampleprep Summary: | Two different protocols were used for each of the different organisms. see attached documentation |
| Sampleprep Protocol Filename: | goncalog_20210305_191823_PR_CO_BioStat_protocol_ecoli.docx AOS_Extraction_Protocol_09March2020.docx |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | water, methanol and IPA extractions |
| Extract Storage: | -80℃ |
| Sample Resuspension: | D2O |
| Sample Derivatization: | n/a |
| Sample Spiking: | n/a |
Analysis:
| MB Sample ID: | SA162494 |
| Analysis ID: | AN002811 |
| Laboratory Name: | Edison Lab - Complex Carbohydrate Research Center |
| Analysis Type: | NMR |
| Acquisition Date: | jan-4-2019 |
| Software Version: | Bruker - Topspin 4.0.6 |
| Operator Name: | Goncalo Gouveia |
| Detector Type: | FT-NMR |
| Data Format: | bruker |
| Num Factors: | 24 |
| Num Metabolites: | 49 |
| Units: | peak height |
NMR:
| NMR ID: | NM000201 |
| Analysis ID: | AN002811 |
| Instrument Name: | 800Mhz Avance III HD |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| NMR Comments: | processing parameter and acquisition can be found on the data |
| Spectrometer Frequency: | 800 |
| NMR Probe: | TCI - cryo-probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.7 mm |
| Shimming Method: | TopShim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | presat |
| Number Of Scans: | 128 |
| Dummy Scans: | 8 |
| Spectral Width: | 20 ppm |
| Line Broadening: | 2 |
| Apodization: | Exponential |
| Baseline Correction Method: | Polynomial order 1 |
| Chemical Shift Ref Std: | 0.00 ppm DSS |
| Binned Increment: | na |
| Binned Data Protocol File: | na |
| Binned Data Excluded Range: | na |
