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MB Sample ID: SA174243
| Local Sample ID: | Dataset_S4 |
| Subject ID: | SU001934 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens;Mus musculus |
| Taxonomy ID: | 9606;10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001934 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens;Mus musculus |
| Taxonomy ID: | 9606;10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Dataset_S4 | SA174243 | FL021377 | PDX GBM - mouse brain tumor section | Tissue Type |
Collection:
| Collection ID: | CO001927 |
| Collection Summary: | As stated in the msiPL paper: Briefly, 12 µm thickness prostate sample diagnosed with a Gleason score of (3+4)=7 were mounted on a microscopy glass slide and coated with CHCA (5 mg/mL in 70/30 methanol/water with 0.1% trifluoroacetic acid V/V) using an automated sprayer (TM-Sprayer, HTX Imaging, Carrboro, NC). The analysis of the samples was performed on a 9.4 Tesla SolariX XR FT ICR mass spectrometer (Bruker Daltonics, Billerica, MA) using the MALDI source in positive ion mode in the mass range between 250-1000 m/z, with a spatial resolution of 120 µm. |
| Sample Type: | Prostate |
Treatment:
| Treatment ID: | TR001946 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP001940 |
| Sampleprep Summary: | As stated in the msiPL paper: For the Prostate tissue: Briefly, 12 µm thickness prostate sample diagnosed with a Gleason score of (3+4)=7 were mounted on a microscopy glass slide and coated with CHCA (5 mg/mL in 70/30 methanol/water with 0.1% trifluoroacetic acid V/V) using an automated sprayer (TM-Sprayer, HTX Imaging, Carrboro, NC). The analysis of the samples was performed on a 9.4 Tesla SolariX XR FT ICR mass spectrometer (Bruker Daltonics, Billerica, MA) using the MALDI source in positive ion mode in the mass range between 250-1000 m/z, with a spatial resolution of 120 µm. For the PDX GBM mouse brain dataset: The intracranial tumor belonging to a PDX model of GBM12 (PDX National Resource, Mayo Clinic), was analyzed by MALDI FT ICR MSI using a 9.4 Tesla SolariX mass spectrometer (Bruker Daltonics, Billerica, MA), using continuous accumulation of selected ions in the mass range between 380-620 m/z. The indium tin oxide (ITO)-coated slide with 12 µm thickness tissue sections , was coated with DHB (160 mg/mL in a 70/30 v/v solution of methanol/0.2% TFA), according to Randall et al2. The 3D MSI dataset was collected from 4 tissue sections with an inter-slice distance of 160 µm. Internal online calibration was performed using heme m/z 616.1776 during data acquisition. |
Combined analysis:
| Analysis ID | AN003010 |
|---|---|
| Analysis type | MS |
| Chromatography type | Unspecified |
| Chromatography system | none |
| Column | none |
| MS Type | MALDI |
| MS instrument type | FT-ICR |
| MS instrument name | Bruker Solarix FT-ICR-MS |
| Ion Mode | POSITIVE |
| Units | Da |
Chromatography:
| Chromatography ID: | CH002231 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | Unspecified |
MS:
| MS ID: | MS002799 |
| Analysis ID: | AN003010 |
| Instrument Name: | Bruker Solarix FT-ICR-MS |
| Instrument Type: | FT-ICR |
| MS Type: | MALDI |
| MS Comments: | Bruker software |
| Ion Mode: | POSITIVE |
