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MB Sample ID: SA189424
| Local Sample ID: | 236_023 |
| Subject ID: | SU002104 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002104 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 236_023 | SA189424 | FL023253 | 2 | Year |
Collection:
| Collection ID: | CO002097 |
| Collection Summary: | Samples were aliquoted from pooled sera collected from multiple individuals. Samples were purchased from Innovative Research (Novi, MI). |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
| Storage Vials: | Wheaton 2mL Cryovials |
| Additives: | Na Heparin |
Treatment:
| Treatment ID: | TR002116 |
| Treatment Summary: | Samples were not treated, beyond the initial aliquotting of the pooled human sera upon receipt at our laboratory before storage at -80C |
Sample Preparation:
| Sampleprep ID: | SP002110 |
| Sampleprep Summary: | Prior to spectral acquisition samples were methanol precipitated and ultrafiltered to remove macromolecules prior to spectra acquisition |
| Sampleprep Protocol Filename: | Serum_Precipitation_SOP.docx, Serum_Filtration_SOP.docx |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Methanol Precipitation |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Samples were resuspended in 50mM phosphate buffer in D2O |
| Sample Spiking: | Samples were spiked with a Formate solution in D2O of known concentration |
Analysis:
| MB Sample ID: | SA189424 |
| Analysis ID: | AN003292 |
| Analysis Type: | NMR |
| Num Factors: | 3 |
| Num Metabolites: | 31 |
| Units: | uM |
NMR:
| NMR ID: | NM000226 |
| Analysis ID: | AN003292 |
| Instrument Name: | Varian 500 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 500 |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Auto shim (gradient shimming), with manual shimming when necessary |
| Pulse Sequence: | 1D-NOESY |
| Water Suppression: | Saturation at 80Hz induced field strength |
| Temperature: | 25 |
| Number Of Scans: | 32 |
