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MB Sample ID: SA268854
| Local Sample ID: | Fig2 N1 30 sec KCl |
| Subject ID: | SU002803 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002803 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Fig2 N1 30 sec KCl | SA268854 | FL035168 | 30 sec KCl | Treatment |
Collection:
| Collection ID: | CO002796 |
| Collection Summary: | Hippocampal slices were stimulated with 50mM potassium chloride over a time course of 5 minutes. The experiment was terminated with thermal preservation at the indicated time points. |
| Sample Type: | Brain |
Treatment:
| Treatment ID: | TR002812 |
| Treatment Summary: | 50mM potassium chloride |
Sample Preparation:
| Sampleprep ID: | SP002809 |
| Sampleprep Summary: | At the specific time chosen for thermal preservation, the slices were flash heated to 80°C for 2 seconds and then cooled to 4°C; the slices were then flash-frozen to -20 °C. The brain slices were stored at -80°C for subsequent thin sectioning in a cryomicrotome. The thin sections were mounted onto conductive glass slides (ITO slides) and sprayed with matrix (1,5-diaminonaphthalene). |
Combined analysis:
| Analysis ID | AN004374 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | MALDI |
| MS instrument type | QTOF |
| MS instrument name | Bruker timsTOF fleX |
| Ion Mode | NEGATIVE |
| Units | a.u. |
Chromatography:
| Chromatography ID: | CH003280 |
| Instrument Name: | none |
| Column Name: | none |
| Column Temperature: | none |
| Flow Gradient: | none |
| Flow Rate: | none |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS004123 |
| Analysis ID: | AN004374 |
| Instrument Name: | Bruker timsTOF fleX |
| Instrument Type: | QTOF |
| MS Type: | MALDI |
| MS Comments: | Mass spectra were acquired on a timsTOF fleX in negative ion mode. Hippocampal sections were outlined as individual ROIs with a pixel size of 20 µm. Most metabolites were acquired in a single imaging run (m/z range 50 - 1000). Each MSI pixel consisted of 1000 laser shots. The laser Smartbeam parameter was set to single with a frequency of 10 kHz. |
| Ion Mode: | NEGATIVE |
