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MB Sample ID: SA268863
Local Sample ID: | Fig3 N5 35min 13C Gluc KCl |
Subject ID: | SU002803 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002803 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Fig3 N5 35min 13C Gluc KCl | SA268863 | FL035170 | 35min 13C Gluc KCl | Treatment |
Collection:
Collection ID: | CO002796 |
Collection Summary: | Hippocampal slices were stimulated with 50mM potassium chloride over a time course of 5 minutes. The experiment was terminated with thermal preservation at the indicated time points. |
Sample Type: | Brain |
Treatment:
Treatment ID: | TR002812 |
Treatment Summary: | 50mM potassium chloride |
Sample Preparation:
Sampleprep ID: | SP002809 |
Sampleprep Summary: | At the specific time chosen for thermal preservation, the slices were flash heated to 80°C for 2 seconds and then cooled to 4°C; the slices were then flash-frozen to -20 °C. The brain slices were stored at -80°C for subsequent thin sectioning in a cryomicrotome. The thin sections were mounted onto conductive glass slides (ITO slides) and sprayed with matrix (1,5-diaminonaphthalene). |
Combined analysis:
Analysis ID | AN004374 |
---|---|
Analysis type | MS |
Chromatography type | None (Direct infusion) |
Chromatography system | none |
Column | none |
MS Type | MALDI |
MS instrument type | QTOF |
MS instrument name | Bruker timsTOF fleX |
Ion Mode | NEGATIVE |
Units | a.u. |
Chromatography:
Chromatography ID: | CH003280 |
Instrument Name: | none |
Column Name: | none |
Column Temperature: | none |
Flow Gradient: | none |
Flow Rate: | none |
Solvent A: | none |
Solvent B: | none |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS004123 |
Analysis ID: | AN004374 |
Instrument Name: | Bruker timsTOF fleX |
Instrument Type: | QTOF |
MS Type: | MALDI |
MS Comments: | Mass spectra were acquired on a timsTOF fleX in negative ion mode. Hippocampal sections were outlined as individual ROIs with a pixel size of 20 µm. Most metabolites were acquired in a single imaging run (m/z range 50 - 1000). Each MSI pixel consisted of 1000 laser shots. The laser Smartbeam parameter was set to single with a frequency of 10 kHz. |
Ion Mode: | NEGATIVE |