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MB Sample ID: SA272159
| Local Sample ID: | Nestin Cre 2-1N |
| Subject ID: | SU002810 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002810 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Nestin Cre 2-1N | SA272159 | FL035253 | Nes-Cre+ | Genotype |
Collection:
| Collection ID: | CO002803 |
| Collection Summary: | Mouse sub ventricular zone tissue was micro dissected and frozen in liquid nitrogen and stored in -80C till further processing. |
| Collection Protocol Filename: | SVZ_Dissection_Protocol-Roychaudhuri_et_al.pdf |
| Sample Type: | sub ventricular zone tissue |
Treatment:
| Treatment ID: | TR002819 |
| Treatment Summary: | No treatment was performed. Nestin -cre+ mice used in the study are conditional knockout for Serine Racemase. |
| Treatment Protocol Filename: | Treatment_Protocol-Roychaudhuri_et_al.pdf |
Sample Preparation:
| Sampleprep ID: | SP002816 |
| Sampleprep Summary: | SVZ Tissues were micro dissected according to protocol. |
| Sampleprep Protocol Filename: | SVZ_Dissection_Protocol-Roychaudhuri_et_al.pdf |
Combined analysis:
| Analysis ID | AN004385 | AN004386 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker micrOTOF-Q II QTOF | Bruker micrOTOF-Q II QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity | intensity |
Chromatography:
| Chromatography ID: | CH003290 |
| Methods Filename: | MS_Metadata-Roychaudhuri_et_al.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 42 |
| Flow Gradient: | 25 min gradient separation (0 min – 2% MPB, 250 μL/min; 3.0 min – 5% MPB, 250 μL/min; 8.0 min – 30% MPB, 250 μL/min; 21 min – 95% MPB, 230 μL/min; 25 min – 95% MPB, 230 μL/min); and 10 min re-equilibration (0 min – 95% MPB, 230 μL/min; 2 min – 5% MPB, 250 μL/min; 9.95 min – 5% MPB, 250 μL/min; 10 min – 5% MPB, 50 μL/min). |
| Flow Rate: | 250 microliter/min |
| Solvent A: | Nova MT MixA: 10 mM ammonium formate in 50:40:10 methanol/acetonitrile/water |
| Solvent B: | Nova MT MixB: 10 mM ammonium formate in 95:5 isopropanol/water |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004134 |
| Analysis ID: | AN004385 |
| Instrument Name: | Bruker micrOTOF-Q II QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | m/z 150-1500 range Lipid features were extracted and aligned using NovaMT LipidScreener. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | MS_Metadata-Roychaudhuri_et_al.pdf |
| MS ID: | MS004135 |
| Analysis ID: | AN004386 |
| Instrument Name: | Bruker micrOTOF-Q II QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | m/z 150-1500 range Lipid features were extracted and aligned using NovaMT LipidScreener. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | MS_Metadata-Roychaudhuri_et_al.pdf |
