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MB Sample ID: SA003712

Local Sample ID:UPALL0001
Subject ID:SU000090
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:approx. 15-16 weeks
Gender:male and female
Animal Animal Supplier:The Jackson Laboratory
Animal Feed:high fat, low fat or normal chow
Species Group:Mammal

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN000111 AN000112
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 QTOF Waters Synapt G2 QTOF
Ion Mode POSITIVE NEGATIVE
Units Normalized abundance Normalized abundance

Chromatography:

Chromatography ID:CH000077
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Pressure:6000-10000
Column Temperature:40 C
Flow Gradient:Time(min) Flow Rate %A %B: 1. Initial 0.400 99.0 1.0; 2. 1.00 0.400 99.0 1.0; 3. 12.00 0.400 1.0 99.0; 4. 14.00 0.400 1.0 99.0; 5. 15.00 0.400 99.0 1.0; 6. 19.00 0.400 99.0 1.0
Flow Rate:0.4 mL/min
Injection Temperature:8 C
Internal Standard:L-Tryptophan-d5
Solvent A:50% acetonitrile/50% water; 0.1% formic acid; 10 mM ammonium acetate
Solvent B:50% acetonitrile/50% water; 0.1% formic acid in 10 mM ammonium acetate
Analytical Time:19 min
Weak Wash Solvent Name:5%MeOH
Weak Wash Volume:1000 uL
Strong Wash Solvent Name:80%MeOH
Strong Wash Volume:1000 uL
Target Sample Temperature:8 C
Sample Loop Size:10 uL
Sample Syringe Size:100 uL
Randomization Order:Yes
Chromatography Type:HILIC
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