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MB Sample ID: SA006528
| Local Sample ID: | F36 |
| Subject ID: | SU000140 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 20 weeks |
| Weight Or Weight Range: | 26.8 +/- 2.2 g |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Labs (Bar Harbor, ME) |
| Animal Housing: | 8 / cage |
| Animal Light Cycle: | 12 h / 12 h light/dark |
| Animal Feed: | Standard chow, ad lib. |
| Animal Water: | Ad lib. Access |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000203 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1200 |
| Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Agilent 6220 TOF |
| Ion Mode | NEGATIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000135 |
| Chromatography Summary: | Polar metabolites were analyzed by HILIC-MS using an aminopropyl stationary phase |
| Instrument Name: | Agilent 1200 |
| Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
| Column Pressure: | 150 bar |
| Column Temperature: | 25 C |
| Flow Gradient: | 80-0% B over 20 minutes, hold 4 minutes, return to 80%B |
| Flow Rate: | 0.25 mL/min |
| Sample Injection: | 25 uL |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 32 min |
| Chromatography Type: | HILIC |
