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MB Sample ID: SA009086
| Local Sample ID: | 1_1 |
| Subject ID: | SU000185 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Combined analysis:
| Analysis ID | AN000259 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890 GC |
| Column | |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000183 |
| Chromatography Summary: | For gas chromatographymass spectrometry (GC-MS), plasma (100 ?L) was extracted using a 900-?L methanol:water (8:1, v/v) mixture containing 5 ?g internal standard, myristic-d27 acid, at ambient temperature 33. Supernatant (900 ?L) was transferred and completely dried in a vacuum concentrator. Subsequently, the tubes were methoximated and derivatized and then analyzed using an Agilent 6890 GC oven with Agilent 5973 MS 34. Sample Treatment and Instrumental Conditions for 1H NMR Metabolomic Analysis For nuclear magnetic resonance (NMR) imaging, plasma (60 ?L) was diluted 140 ?L with 0.2M phosphate buffer (pH 7.4):D2O containing a mixture of 16 mM formate and 4 mM TSP (1:1, v/v). After filtering (0.22 ?m), the samples were transferred into a 3-mm-diameter NMR tube. High-resolution 1H NMR spectra were acquired at 600 MHz on a Bruker Avance III 600 spectrometer. Spectra peaks were identified according to Chenomx NMR Suite 6.1 software and data in the literature 35, 36. |
| Instrument Name: | Agilent 6890 GC |
| Chromatography Type: | GC |
