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MB Sample ID: SA009177
| Local Sample ID: | ms2418-16 |
| Subject ID: | SU000187 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000262 |
|---|---|
| Analysis type | MS |
| Chromatography type | |
| Chromatography system | Waters Acquity |
| Column | |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantum Ultra |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH000185 |
| Chromatography Summary: | Amino acids in particular are relatively stable for several years, with the exception of their keto-acids (e.g., KIC), which we have not included in the present analysis. Subsequently, we separated the samples using an Acquity UPLC system, followed by mass detection using a TSQ Ultra Quantum from Thermo Finnigan running in ESI positive mode as previously described [32]. We calculated the AA and AA metabolites concentrations using 10 point standard curves as previously described [32]. We handled the pre- and post-intervention samples similarly and analyzed them in the same run. The reproducibility of the UPLC/MS/MS method in the MCRMC is excellent (average CV ~ 9.5%) [32]. |
| Instrument Name: | Waters Acquity |
