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MB Sample ID: SA011055
| Local Sample ID: | MaleRat8 |
| Subject ID: | SU000256 |
| Subject Type: | Human/animal |
| Subject Species: | Homo sapiens;Rattus norvegicus |
| Taxonomy ID: | 9606;10116 |
| Genotype Strain: | Sprague-Dawley (Rat) |
| Age Or Age Range: | 23-29 (Human) |
| Weight Or Weight Range: | 220-250g (Rat) |
| Gender: | Male/Female (Human and Rat) |
| Human Race: | Yellow Race (Human) |
| Human Inclusion Criteria: | Post-graduate students (Human) |
| Animal Housing: | pathogen-free animal facility (Rat) |
| Animal Light Cycle: | 12h light/dark cycle (Rat) |
| Animal Feed: | free access to standard food (Rat) |
| Animal Water: | free access to water (Rat) |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000357 | AN000358 | AN000359 | AN000360 | AN000361 | AN000362 |
|---|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Waters Synapt-HDMS | Waters Synapt-HDMS | Waters Synapt-HDMS | Waters Synapt-HDMS | Waters Synapt-HDMS | Waters Synapt-HDMS |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE | NEGATIVE |
| Units | Peak Height | Peak Height | Peak Height | Peak Height | Peak Height | Peak Height |
Chromatography:
| Chromatography ID: | CH000264 |
| Chromatography Summary: | Untargeted UPLC-TOFMS |
| Methods Filename: | 3_CHR_PROT.pdf |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 24? |
| Flow Gradient: | Isocratic 2% B (01 min), linear gradient from 2% to 5% B (12 min), 5% to 12% B (25 min), 12% to 20% B (510 min), 20% to 30% B (1012 min), 30% to 50% B (1213 min), 50% to 100% B (1315 min), isocratic 100% B for 1min, then back to 2% B in 1 min and isocratic 2% B for 3min before next run |
| Flow Rate: | 0.5 ml/min |
| Injection Temperature: | 24? |
| Internal Standard: | Amygdalin |
| Sample Injection: | 8?L |
| Sampling Cone: | 40V |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 20min |
| Capillary Voltage: | Positive ion mode:3kV; Negative ion mode: 2.9kV |
| Chromatography Type: | Reversed phase |
