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MB Sample ID: SA011335
| Local Sample ID: | folch_low |
| Subject ID: | SU000266 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC - Supplier |
| Subject Comments: | P?+10 |
| Cell Primary Immortalized: | immortalized |
| Cell Passage Number: | P?+10 |
| Cell Counts: | P?+10 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000384 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Supelco Analytical Titan C18 |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH000276 |
| Chromatography Summary: | Reversed Phase |
| Methods Filename: | m_lipid_pos_switch_100 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Supelco Analytical Titan C18 |
| Column Pressure: | 1241 bar (max) |
| Column Temperature: | 30 C |
| Flow Gradient: | 32% B at 0 min, 40% B at 1 min, a hold at 40% B until 1.5 min, 45% B at 4 min, 50% B at 5 min, 60% B at 8 min, 70% B at 11 min, and 80% B at 14 min |
| Flow Rate: | 0.5 mL/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 2 μL |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
| Analytical Time: | 21 min |
| Randomization Order: | 3,10,12,11,8,6,5,4,7,9,2,1 |
| Chromatography Type: | Reversed phase |
