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MB Sample ID: SA011357
| Local Sample ID: | 6C |
| Subject ID: | SU000267 |
| Subject Type: | Bacterial cells |
| Subject Species: | Staphylococcus aureus |
| Taxonomy ID: | 1280 |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000387 | AN000388 | AN000389 | AN000390 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Agilent 1290 Infinity |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters AccQ Tag (100 x 2mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Thermo Quantiva QQQ | Thermo Quantiva QQQ | Thermo Quantiva QQQ | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | nmol/mg protein | nmol/mg protein | nmol/mg protein | nmol/mg protein |
Chromatography:
| Chromatography ID: | CH001331 |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001332 |
| Methods Filename: | SB_NAD_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
| Column Temperature: | 30 |
| Flow Rate: | 0.65 mL/min |
| Solvent A: | 100% water; 10 mM ammonium acetate, pH 9.5 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001333 |
| Methods Filename: | SB_NADH_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001334 |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
