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MB Sample ID: SA013981
| Local Sample ID: | Naive WT 3 042215 |
| Subject ID: | SU000327 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Animal Animal Supplier: | WT (CRBN +/+) mice are from Jackson labs and CRBN -/- mice have been bred at Moffitt Cancer Center |
| Animal Housing: | Standard bedding + cotton pads, hepa filtered air |
| Animal Light Cycle: | Dark 6pm-6am |
| Animal Feed: | Feed from Harlan, not sterile |
| Animal Water: | autoclaved H2O |
| Animal Inclusion Criteria: | Animals used in this experiment were sex-matched and age matched. The ages were 8-12 weeks old in both sets of experiments |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000486 | AN000487 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000347 |
| Methods Filename: | Metabolomics_LCMSProtocol.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
