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MB Sample ID: SA025725
Local Sample ID: | Sa_pre_43 |
Subject ID: | SU000517 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
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Combined analysis:
Analysis ID | AN000762 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu GCMS-QP2010 ultra |
Column | a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Shimadzu QP2010 Ultra |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH000548 |
Chromatography Summary: | The device used was GCMS-QP2010 ultra (Shimadzu Inc., Kyoto, Japan) equipped with a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA) and AOC-20i (Shimadzu) as an autosampler. The injection temperature was 230 °C. The helium gas flow rate through the column was 1.12 ml/min. The column temperature was held at 80 °C for 2 min and then raised by 15 °C /min to 330 °C and held for 6 min. The transfer line and the ion source temperatures were 250 °C and 200 °C, respectively. Ions were generated by a 70 kV electron impact (EI), and 20 scans/sec were recorded over the mass range 85-500. A standard alkane mixture (C8-C40) was injected at the beginning of the analysis for subsequent identification of metabolites. |
Instrument Name: | Shimadzu GCMS-QP2010 ultra |
Column Name: | a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA) |
Column Temperature: | The column temperature was held at 80 °C for 2 min and then raised by 15 °C /min to 330 °C and held for 6 min. |
Flow Rate: | The helium gas flow rate through the column was 1.12 ml/min. |
Sample Injection: | 1 µL |
Chromatography Type: | GC |