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MB Sample ID: SA028716
Local Sample ID: | 319819 |
Subject ID: | SU000574 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male |
Human Race: | White |
Human Inclusion Criteria: | blunt trauma without isolated head injury, defined as either abbreviated injury score (AIS) head <4 OR Glasgow Coma Scale (GCS) motor >3 within 24 hours of injury, emergency department arrival within 6 hours from time of injury, blood transfusion within 12 hours of injury, base deficit of at least 6 or systolic blood pressure <90 mmHg within 60 minutes of emergency department arrival, and fully or partially intact cervical spinal cord |
Human Exclusion Criteria: | age <16 years, anticipated survival of <24 hours from injury, anticipated survival <28 days due to pre-existing medical condition, inability to obtain first blood draw within first 12 hours after injury, traumatic brain injury (i.e., GCS ≤8 after ICU admission AND brain computerized tomography scan abnormality within 12 hours after injury), immunosuppression, significant pre-existing organ dysfunction, and receiving home oxygen therapy |
Species Group: | Human |
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Combined analysis:
Analysis ID | AN000843 | AN000844 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific Q-Exactive | Thermo Scientific Q-Exactive |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000603 |
Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Instrument Name: | Thermo Scientific Q-Exactive |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |