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MB Sample ID: SA069159
Local Sample ID: | V1E2T1N |
Subject ID: | SU001071 |
Subject Type: | Other |
Subject Species: | Xenopus laevis |
Taxonomy ID: | 8355 |
Age Or Age Range: | Embryos were obtained from natural mating of frogs (Nasco) |
Weight Or Weight Range: | Sexually mature male and female frogs |
Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001692 | AN001693 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | CE | CE |
Chromatography system | Custom-built CE system | Custom-built CE system |
Column | Bare fused silica capillary | Bare fused silica capillary |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Bruker Impact HD | Bruker Impact HD |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH001191 |
Chromatography Summary: | Metabolites were separated in a custom-built capillary electrophoresis (CE) system. |
Instrument Name: | Custom-built CE system |
Column Name: | Bare fused silica capillary |
Column Temperature: | Room temperature |
Injection Temperature: | Room temperature |
Sample Injection: | Ca. 10 nL |
Solvent A: | 100% water; 1% formic acid |
Analytical Time: | 45 min of separation |
Capillary Voltage: | During cationic separation, +19,000-20,000 V was applied on the inlet end of the CE capillary. |
Preconditioning: | Sodium hydroxide solution |
Sheath Liquid: | During cationic analysis, the electrospray sheath solution was 50% methanol with 0.1% formic acid. |
Chromatography Type: | CE |
Chromatography ID: | CH001192 |
Chromatography Summary: | Metabolites were separated in a custom-built capillary electrophoresis (CE) system. |
Instrument Name: | Custom-built CE system |
Column Name: | Bare fused silica capillary |
Column Temperature: | Room temperature |
Injection Temperature: | Room temperature |
Sample Injection: | Ca. 10 nL |
Solvent A: | 100% water; 20 mM ammonium bicarbonate |
Analytical Time: | 45 min of separation |
Capillary Voltage: | During anionic, +19,000-20,000 V was applied on the inlet end of the CE capillary. |
Preconditioning: | Sodium hydroxide solution |
Sheath Liquid: | During cationic analysis, the electrospray sheath solution was 0.2 mM ammonium bicarbonate in 50% isopropanol. |
Chromatography Type: | CE |