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MB Sample ID: SA069160
| Local Sample ID: | V1E2T1P |
| Subject ID: | SU001071 |
| Subject Type: | Other |
| Subject Species: | Xenopus laevis |
| Taxonomy ID: | 8355 |
| Age Or Age Range: | Embryos were obtained from natural mating of frogs (Nasco) |
| Weight Or Weight Range: | Sexually mature male and female frogs |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001692 | AN001693 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Custom-built CE system | Custom-built CE system |
| Column | Bare fused silica capillary | Bare fused silica capillary |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker Impact HD | Bruker Impact HD |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001191 |
| Chromatography Summary: | Metabolites were separated in a custom-built capillary electrophoresis (CE) system. |
| Instrument Name: | Custom-built CE system |
| Column Name: | Bare fused silica capillary |
| Column Temperature: | Room temperature |
| Injection Temperature: | Room temperature |
| Sample Injection: | Ca. 10 nL |
| Solvent A: | 100% water; 1% formic acid |
| Analytical Time: | 45 min of separation |
| Capillary Voltage: | During cationic separation, +19,000-20,000 V was applied on the inlet end of the CE capillary. |
| Preconditioning: | Sodium hydroxide solution |
| Sheath Liquid: | During cationic analysis, the electrospray sheath solution was 50% methanol with 0.1% formic acid. |
| Chromatography Type: | CE |
| Chromatography ID: | CH001192 |
| Chromatography Summary: | Metabolites were separated in a custom-built capillary electrophoresis (CE) system. |
| Instrument Name: | Custom-built CE system |
| Column Name: | Bare fused silica capillary |
| Column Temperature: | Room temperature |
| Injection Temperature: | Room temperature |
| Sample Injection: | Ca. 10 nL |
| Solvent A: | 100% water; 20 mM ammonium bicarbonate |
| Analytical Time: | 45 min of separation |
| Capillary Voltage: | During anionic, +19,000-20,000 V was applied on the inlet end of the CE capillary. |
| Preconditioning: | Sodium hydroxide solution |
| Sheath Liquid: | During cationic analysis, the electrospray sheath solution was 0.2 mM ammonium bicarbonate in 50% isopropanol. |
| Chromatography Type: | CE |
