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MB Sample ID: SA085197
Local Sample ID: | B4 |
Subject ID: | SU001277 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002014 | AN002015 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 | Agilent 1290 |
Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um,130Å) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um,130Å) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | ABI Sciex 5600 TripleTOF | ABI Sciex 5600 TripleTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Normalized intensities | Normalized intensities |
Chromatography:
Chromatography ID: | CH001457 |
Chromatography Summary: | Analyses were performed by Agilent 1290 Series UHPLC instrument coupled to Sciex TripleTOF 5600+ MS with duospray source and Pal HTC-XS autosampler from CTC. Positive and negative ESI ionization were used in separate LC-MS runs. Conditions: Acquity UPLC CSH C18 (130Å, 1.7 µm, 2.1 mm X 100 mm) column was utilized with Acquity UPLC CSH C18 VanGuard pre-column (130Å, 1.7 µm, 2.1 mm X 5 mm). The mobile phase was composed of 10 mM ammonium formate and 0.1 % formic acid dissolved in 60:40 ACN:H2O (v/v) (A) and 90:10 (v/v) IPA:ACN (B). |
Methods Filename: | ccalcas_LC-MS.pdf |
Instrument Name: | Agilent 1290 |
Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um,130Å) |
Column Temperature: | 65 |
Flow Rate: | 600 uL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |