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MB Sample ID: SA099945

Local Sample ID:20181222-OJ-SWATH-5
Subject ID:SU001448
Subject Type:Other
Subject Species:Malus domestica;Citrus sinensis
Taxonomy ID:3750;2711

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Combined analysis:

Analysis ID AN002294
Analysis type MS
Chromatography type Reversed phase
Chromatography system SCIEX ExionLC
Column Agilent Eclipse Plus C18,RRHD (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name ABI Sciex 6600 TripleTOF
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH001685
Chromatography Summary:All samples were subjected to reversed-phase chromatography using a UHPLC system, including ExionLC solvent valve, ExionLC AD pump, ExionLC autosampler, ExionLC controller, ExionLC AC column oven (SCIEX, Redwood City, CA, USA). Mobile phases A was 0.2% formic acid in water and B was acetonitrile, respectively. Chromatographic separation was performed at a flow rate of 300 μL/min using a UHPLC column (Eclipse Plus C18, RRHD 1.8 μm, 2.1*100 mm, Agilent, USA) maintained at 40 °C. Solvent gradient was as follow: 0−10 min, 5%−35% B; 10−12 min, 35−100% B; 12−15 min, 100% B; 15−15.01 min, 100%−5% B; 15.01−18 min, 5% B. Injection volume was 2 μL for all samples.
Instrument Name:SCIEX ExionLC
Column Name:Agilent Eclipse Plus C18,RRHD (100 x 2.1mm,1.8um)
Flow Gradient:0-10 min, 5%-35% B; 10-12 min, 35-100% B; 12-15 min, 100% B; 15-15.01 min, 100%-5% B; 15.01-18 min,
Flow Rate:300ul/min
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase
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