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MB Sample ID: SA118533

Local Sample ID:ArcA_1
Subject ID:SU001495
Subject Type:Bacteria
Subject Species:Escherichia coli
Taxonomy ID:562
Genotype Strain:K-12 MG1655

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Combined analysis:

Analysis ID AN002376 AN002377
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE NEGATIVE
Units umol/gDCW and Height ratio/gDCW umol/gDCW

Chromatography:

Chromatography ID:CH001744
Chromatography Summary:The detailed chromatography and MS protocol has been described in "MS-method_msi.pdf" file.
Methods Filename:ankita_pal_MS-method_msi.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
Flow Rate:200 ul/min
Solvent A:90% acetonitrile/10% water; 10 mM ammonium acetate,pH 9.23
Solvent B:10% acetonitrile/90% water; 10 mM ammonium acetate,pH 9.23
Chromatography Type:HILIC
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