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MB Sample ID: SA125770
| Local Sample ID: | 97 |
| Subject ID: | SU001565 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002470 | AN002471 | AN002472 | AN002473 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) | Hypersil GOLD (150 x 2.1mm,1.9um) | Hypersil GOLD (150 x 2.1mm,1.9um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | MS count | MS Counts | MS Counts | MS Counts |
Chromatography:
| Chromatography ID: | CH001810 |
| Chromatography Summary: | HILIC experiments were performed using a ZIC-HILIC column 2.1x100 mm, 3.5μm, 200Å (Merck Millipore) and mobile phase solvents consisting of 10mM ammonium acetate in 50/50 acetonitrile/water (A) and 10 mM ammonium acetate in 95/5 acetonitrile/water (B).(Contrepois et al., 2015) |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.5 ml/min |
| Solvent A: | 95% acetonitrile/5% water; 10 mM ammonium acetate |
| Solvent B: | 95% acetonitrile/5% water; 10 mM ammonium acetate |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001811 |
| Chromatography Summary: | RPLC experiments were performed using a Hypersil GOLD column 2.1 x 150 mm, 1.9 µm, 175Å (Thermo Scientific) and mobile phase solvents consisting of 0.06% acetic acid in water (A) and 0.06% acetic acid in methanol (B). (Contrepois et al., 2015) |
| Chromatography Comments: | Hypersil GOLD column 2.1 x 150 mm, 1.9 µm, 175Å (Thermo Scientific) |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Hypersil GOLD (150 x 2.1mm,1.9um) |
| Column Temperature: | 60 |
| Flow Rate: | 0.6 ml/min |
| Solvent A: | 100% water; 0.06% acetic acid |
| Solvent B: | 100% methanol; 0.06% acetic acid |
| Chromatography Type: | Reversed phase |
