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MB Sample ID: SA160356

Local Sample ID:1219
Subject ID:SU001789
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:6–8 weeks old
Gender:Female
Animal Animal Supplier:Charles River Frederick Research Model Facility

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Combined analysis:

Analysis ID AN002787
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 6545 QTOF-MS
Column AdvanceBio Glycan Map (2.1 x 100mm, 2.7µm)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6540 QTOF
Ion Mode NEGATIVE
Units arbitrary units

Chromatography:

Chromatography ID:CH002062
Instrument Name:Agilent 6545 QTOF-MS
Column Name:AdvanceBio Glycan Map (2.1 x 100mm, 2.7µm)
Column Temperature:35
Flow Gradient:100% B, 0.5 min; 95% B, 2.0 min; 60% B, 3.0 min; 35% B, 5 min; hold 0.25 min; 0% B, 6 min; hold 0.5 min; 100% B, 7.8 min; equilibrate for 1.7 min.
Flow Rate:0.2 mL/min
Solvent A:88% water/12% acetonitrile; 10 mM ammonium acetate; titrated with formic acid and ammonium hydroxide to pH 6.85
Solvent B:90% acetonitrile/10% water; 10 mM ammonium acetate; titrated with formic acid and ammonium hydroxide to pH 6.85
Chromatography Type:HILIC
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