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MB Sample ID: SA161874
| Local Sample ID: | Mito_007_po |
| Subject ID: | SU001794 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | HeLa cell line |
| Gender: | Not applicable |
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Combined analysis:
| Analysis ID | AN002797 | AN002798 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | ABI Sciex 5600 TripleTOF | ABI Sciex 5600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH002067 |
| Chromatography Summary: | Chromatographic separation of lipids in cells and mitochondria was performed with an Acquity UPLC system (Waters, USA) using an Acquity UPLC CSH C18 column (2.1100 mm, 1.7 µm; Waters) at 55°C and a flow rate of 0.4 ml/min. The mobile phase for positive ion mode comprised 10 mM ammonium formate in water/acetonitrile (40:60, v/v) containing 0.1% formic acid (solvent A) and isopropanol/acetonitrile (90:10, v/v) containing 0.1% formic acid (solvent B). The mobile phase for negative ion mode comprised 10 mM ammonium acetate in water/acetonitrile (60:40, v/v) (solvent A) and isopropanol/acetonitrile (90:10, v/v) (solvent B). The UPLC gradient was programmed as follows: 40% to 43% B from 0 min to 2 min, 43% to 50% B from 2 min to 2.1 min, 50% to 54% B from 2.1 min to 12 min, 54% to 70% B from 12 min to 12.1 min, 70% to 99% B from 12.1 min to 18 min, 99% to 40% B from 18 min to 18.1 min, and 40% B for 2 min to equilibrate for the next run. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 55 |
| Flow Gradient: | 40% to 43% B from 0 min to 2 min, 43% to 50% B from 2 min to 2.1 min, 50% to 54% B from 2.1 min to 12 min, 54% to 70% B from 12 min to 12.1 min, 70% to 99% B from 12.1 min to 18 min, 99% to 40% B from 18 min to 18.1 min, and 40% B for 2 min to equilibrate for the next run. |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 40% water/60acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile |
| Chromatography Type: | Reversed phase |
