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MB Sample ID: SA162941
| Local Sample ID: | VAT (ob)3 |
| Subject ID: | SU001815 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002829 | AN002830 | AN002831 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC |
| Chromatography system | Vanquish Horizon | Vanquish Horizon | Vanquish Horizon |
| Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) | Thermo Accucore C18 (150 x 2.1mm,2.6um) | Waters ACQUITY UPLC BEH HILIC (100 x 1.0mm,1.7um,130 Å) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap; Thermo Fusion Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | UNSPECIFIED | UNSPECIFIED | POSITIVE |
| Units | fmol/μg protein | fmol/μg protein | fmol/μg protein |
Chromatography:
| Chromatography ID: | CH002092 |
| Chromatography Summary: | Three chromatography types were employed to allow for optimal lipidome separation (i.e. C18 RPC for amphiphilic lipids, C30 RPC for triacylglycerols, HILIC for acylcarnitines). All details on the respective methods are provided in the attached methods document |
| Instrument Name: | Vanquish Horizon |
| Column Name: | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0-5 min - 50 to 80 % B (curve 5), 5-22 min - 80 to 95 % B (curve 4), 22-26 min - 95 % isocratic, 26-27 min - 95 to 100 % B (curve 5), 27-47 min - 100 % B isocratic, 47-47.1 min - 100 to 50 % B followed by 8 min re-equilibration at 50% B |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 50% acetonitrile/50% water; 0.1% formic acid; 5 mM ammonium formate |
| Solvent B: | 85% isopropanol/10% acetonitrile/5% water; 0.1% formic acid; 5 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002093 |
| Chromatography Summary: | Three chromatography types were employed to allow for optimal lipidome separation (i.e. C18 RPC for amphiphilic lipids, C30 RPC for triacylglycerols, HILIC for acylcarnitines). All details on the respective methods are provided in the attached methods document |
| Instrument Name: | Vanquish Horizon |
| Column Name: | Thermo Accucore C18 (150 x 2.1mm,2.6um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0-5 min - 50 to 80 % B (curve 5), 5-22 min - 80 to 95 % B (curve 4), 22-26 min - 95 % isocratic, 26-27 min - 95 to 100 % B (curve 5), 27-47 min - 100 % B isocratic, 47-47.1 min - 100 to 50 % B followed by 8 min re-equilibration at 50% B |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 50% acetonitrile/50% water; 0.1% formic acid; 5 mM ammonium formate |
| Solvent B: | 85% isopropanol/10% acetonitrile/5% water; 0.1% formic acid; 5 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002094 |
| Chromatography Summary: | Three chromatography types were employed to allow for optimal lipidome separation (i.e. C18 RPC for amphiphilic lipids, C30 RPC for triacylglycerols, HILIC for acylcarnitines). All details on the respective methods are provided in the attached methods document |
| Instrument Name: | Vanquish Horizon |
| Column Name: | Waters ACQUITY UPLC BEH HILIC (100 x 1.0mm,1.7um,130 Å) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-10 min - 0 to 10 % B (curve 5), 10-10.1 min - 10 to 0 % B (curve 5) followed by 5 min re-equilibration at 0% B |
| Flow Rate: | 0.15 ml/min |
| Solvent A: | 96% acetonitrile/4% water; 5 mM ammonium acetate |
| Solvent B: | 100% water; 5 mM ammonium acetate |
| Chromatography Type: | HILIC |
