Return to study ST002112 main page
MB Sample ID: SA251712
| Local Sample ID: | 324 |
| Subject ID: | SU002601 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004115 | AN004116 | AN004117 | AN004118 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um) | SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um) | Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um) | Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Area | Area | Area | Area |
Chromatography:
| Chromatography ID: | CH003049 |
| Chromatography Summary: | HILIC experiments were performed using a ZIC-HILIC column 2.1x100 mm, 3.5μm, 200Å (Merck Millipore) and mobile phase solvents consisting of 10mM ammonium acetate in 50/50 acetonitrile/water (A) and 10 mM ammonium acetate in 95/5 acetonitrile/water (B).(Contrepois et al., 2015) |
| Instrument Name: | Thermo Vanquish |
| Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um) |
| Column Temperature: | 40 |
| Flow Gradient: | The gradient profile used was 99%B for 2 minutes, 99-1% B over 15 minutes, 1%B for 3 minutes. 99%B for 5 minutes. |
| Flow Rate: | .5 ml/min |
| Solvent A: | 10mM ammonium acetate in 50/50 acetonitrile/water |
| Solvent B: | 10 mM ammonium acetate in 95/5 acetonitrile/water |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003050 |
| Chromatography Summary: | RPLC experiments were performed using a Zorbax SBaq column 2.1 x 50 mm, 1.8 μm, 100Å (Agilent Technologies) and mobile phase solvents consisting of 0.06% acetic acid in water (A) and 0.06% acetic acid in methanol (B). (Contrepois et al., 2015) |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um) |
| Column Temperature: | 60 |
| Flow Gradient: | The gradient profile used was 1% B for 1 minute, 1%-80% B over 8 minutes, 80% B for 3 minutes, 1% B for 5 minutes. |
| Flow Rate: | .6ml/min |
| Solvent A: | 0.06% acetic acid in water |
| Solvent B: | 0.06% acetic acid in methanol |
| Chromatography Type: | Reversed phase |
