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MB Sample ID: SA255498
| Local Sample ID: | Sea ice_T-S_A |
| Subject ID: | SU002639 |
| Subject Type: | Water sample |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004181 | AN004182 | AN004183 | AN004184 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class |
| Column | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS CN (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Estimated metabolite carbon concentration (nmol C per L) | Estimated metabolite carbon concentration (nmol C per L) | Estimated metabolite carbon concentration per particulate carbon (nmol C per umol C) | Estimated metabolite carbon concentration (nmol C per L) |
Chromatography:
| Chromatography ID: | CH003098 |
| Chromatography Summary: | See attached summary. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 100% B for 2 minutes, ramped to 64% A over 18 minutes, ramped up to 100% A over 1 minute, held at 100% A for 7 minutes, and equilibrated at 100% B for 22 minutes (total time is 50 minutes) |
| Flow Rate: | 0.15 mL/min |
| Solvent A: | 10mM ammonium carbonate;85% water/15% acetonitrile |
| Solvent B: | 10mM ammonium carbonate;85% acetonitrile/15% water |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003099 |
| Chromatography Summary: | See attached summary. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Gradient: | Initial conditions were 90% A and 10% B. The column was ramped to 80% B over 33 minutes, ramped up to 90% B over 12 minutes, held at 90% B for 1 minute, and equilibrated to 90%A and 10% B for 6 minutes (total time is 52 minutes). |
| Flow Rate: | 0.45 mL/min |
| Solvent A: | 10mM ammonium formate; 60% acetonitrile/40% water; 0.1% formic acid |
| Solvent B: | 10mM ammonium formate; 90% isopropyl alcohol/10% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003100 |
| Chromatography Summary: | See attached summary. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters ACQUITY UPLC HSS CN (100 x 2.1mm,1.8um) |
| Column Temperature: | 35 |
| Flow Gradient: | The column was held at 5% B for 2 minutes, ramped to 100% B over 18 minutes, held at 100% B for 2 minutes, and equilibrated at 5% B for 5 minutes (total run time is 25 minutes) |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in acetonitrile |
| Chromatography Type: | Reversed phase |
