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MB Sample ID: SA267018
Local Sample ID: | ko5-1p |
Subject ID: | SU002798 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Wild-type and ATP10A knockouts |
Age Or Age Range: | 16-20 weeks |
Weight Or Weight Range: | 23-28 grams |
Animal Housing: | temperature and humidity-controlled facilities |
Animal Light Cycle: | 12 h light/dark cycles |
Animal Feed: | standard chow or 60% HFD (D12492, Research Diets) ad libitum, |
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Combined analysis:
Analysis ID | AN004369 | AN004370 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 6560 | Agilent 6560 |
Column | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6560 Ion Mobility | Agilent 6560 Ion Mobility |
Ion Mode | POSITIVE | NEGATIVE |
Units | Ion Abundances | Ion Abundances |
Chromatography:
Chromatography ID: | CH003275 |
Chromatography Summary: | HPLC-IM-MS/MS on an Agilent 6560 mass spectrometer using a ZORBAX Extend-C18 RPLC column (Phase A: 0.1% formic acid and 10 mM NH4CHOO in water, Phase B: 0.1% formic acid and 10 mM NH4CHOO in 60:36:4 isopropanol:acetonitrile:water). |
Instrument Name: | Agilent 6560 |
Column Name: | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) |
Column Temperature: | 40 |
Flow Gradient: | 70% B for 1 min, 70-86% B in 2.5 min, 86% B for 6.5 min, 86-100% B in 1 min, 100% B for 6 min, 100-70% B in 0.1 min, and 70% B for 1.9 min. |
Flow Rate: | 300 ul/min |
Solvent A: | Water (10mM Amm. Formate) 0.1% formic acid |
Solvent B: | 60:36:4 IPA:ACN:H2O (10mM Amm. Formate) 0.1% formic acid |
Chromatography Type: | Reversed phase |