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MB Sample ID: SA267026
| Local Sample ID: | wt1-2n |
| Subject ID: | SU002798 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Wild-type and ATP10A knockouts |
| Age Or Age Range: | 16-20 weeks |
| Weight Or Weight Range: | 23-28 grams |
| Animal Housing: | temperature and humidity-controlled facilities |
| Animal Light Cycle: | 12 h light/dark cycles |
| Animal Feed: | standard chow or 60% HFD (D12492, Research Diets) ad libitum, |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004369 | AN004370 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 6560 | Agilent 6560 |
| Column | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6560 Ion Mobility | Agilent 6560 Ion Mobility |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Ion Abundances | Ion Abundances |
Chromatography:
| Chromatography ID: | CH003275 |
| Chromatography Summary: | HPLC-IM-MS/MS on an Agilent 6560 mass spectrometer using a ZORBAX Extend-C18 RPLC column (Phase A: 0.1% formic acid and 10 mM NH4CHOO in water, Phase B: 0.1% formic acid and 10 mM NH4CHOO in 60:36:4 isopropanol:acetonitrile:water). |
| Instrument Name: | Agilent 6560 |
| Column Name: | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 70% B for 1 min, 70-86% B in 2.5 min, 86% B for 6.5 min, 86-100% B in 1 min, 100% B for 6 min, 100-70% B in 0.1 min, and 70% B for 1.9 min. |
| Flow Rate: | 300 ul/min |
| Solvent A: | Water (10mM Amm. Formate) 0.1% formic acid |
| Solvent B: | 60:36:4 IPA:ACN:H2O (10mM Amm. Formate) 0.1% formic acid |
| Chromatography Type: | Reversed phase |
