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MB Sample ID: SA272151
| Local Sample ID: | Nestin CreP 2-1 |
| Subject ID: | SU002810 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004385 | AN004386 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker micrOTOF-Q II QTOF | Bruker micrOTOF-Q II QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity | intensity |
Chromatography:
| Chromatography ID: | CH003290 |
| Methods Filename: | MS_Metadata-Roychaudhuri_et_al.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 42 |
| Flow Gradient: | 25 min gradient separation (0 min – 2% MPB, 250 μL/min; 3.0 min – 5% MPB, 250 μL/min; 8.0 min – 30% MPB, 250 μL/min; 21 min – 95% MPB, 230 μL/min; 25 min – 95% MPB, 230 μL/min); and 10 min re-equilibration (0 min – 95% MPB, 230 μL/min; 2 min – 5% MPB, 250 μL/min; 9.95 min – 5% MPB, 250 μL/min; 10 min – 5% MPB, 50 μL/min). |
| Flow Rate: | 250 microliter/min |
| Solvent A: | Nova MT MixA: 10 mM ammonium formate in 50:40:10 methanol/acetonitrile/water |
| Solvent B: | Nova MT MixB: 10 mM ammonium formate in 95:5 isopropanol/water |
| Chromatography Type: | Reversed phase |
