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MB Sample ID: SA001578
| Local Sample ID: | 131221ajcsa16_1 |
| Subject ID: | SU000042 |
| Subject Type: | Insect |
| Subject Species: | Centroptilum triangulifer;Atherix;Megaloptera |
| Taxonomy ID: | 2078957;124299;50553 |
| Species Group: | Insect |
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Collection:
| Collection ID: | CO000025 |
| Collection Summary: | whole insects were collected |
| Collection Protocol Comments: | Original culture material was obtained from the Stroud Water Research Center (Avondale, PA). Larvae were reared at 22C and fed a periphytic diet. When larvae reached a suitable size, they were separated from their food source for 12 hours to evacuate gut contents. Larvae were then divided into two treatment groups a control group maintained at the culturing temperature of 22C, and a thermal challenge group. The thermal challenge group was subjected to a temperature increase at a rate of 1C per hour. This rate of temperature change is commonly observed in temperate streams. When the treatment temperature reached 30C, larvae from both control and treatment groups were flash frozen in liquid nitrogen in groups of 12-13 larvae. Each grouping of 12-13 larvae comprised a composite replicate for the metabolic profiling analysis. A total of 3 replicates each for the control and thermal treatments were used in this study. Some larvae had developed dark wing pads during the experiment. This is a signal that the larvae were very close to emerging from the aquatic larval phase to a winged sub-adult phase. Enough of these larvae were present in the control treatment to be removed from the control cohort and considered as an independent sample. |
| Sample Type: | whole insect |
| Volumeoramount Collected: | 50 mg |
| Storage Conditions: | -80C |
