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MB Sample ID: SA002143
| Local Sample ID: | AD_01 |
| Subject ID: | SU000067 |
| Subject Type: | Insect |
| Subject Species: | Centroptilum triangulifer;Atherix;Megaloptera |
| Taxonomy ID: | 2078957;124299;50553 |
| Species Group: | Insect |
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Collection:
| Collection ID: | CO000050 |
| Collection Summary: | whole insects were collected |
| Collection Protocol Comments: | Original culture material was obtained from the Stroud Water Research Center (Avondale, PA). Larvae were reared at 22°C and fed a periphytic diet. When larvae reached a suitable size, they were separated from their food source for 12 hours to evacuate gut contents. Larvae were then divided into two treatment groups a control group maintained at the culturing temperature of 22°C, and a thermal challenge group. The thermal challenge group was subjected to a temperature increase at a rate of 1°C per hour. This rate of temperature change is commonly observed in temperate streams. When the treatment temperature reached 30°C, larvae from both control and treatment groups were flash frozen in liquid nitrogen in groups of 12-13 larvae. Each grouping of 12-13 larvae comprised a composite replicate for the metabolic profiling analysis. A total of 3 replicates each for the control and thermal treatments were used in this study. Some larvae had developed dark wing pads during the experiment. This is a signal that the larvae were very close to emerging from the aquatic larval phase to a winged sub-adult phase. Enough of these larvae were present in the control treatment to be removed from the control cohort and considered as an independent sample. |
| Sample Type: | whole insect |
| Volumeoramount Collected: | 50 mg |
| Storage Conditions: | -80C |
