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MB Sample ID: SA014973
Local Sample ID: | 27327 |
Subject ID: | SU000355 |
Subject Type: | Animal |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
Species Group: | Mammals |
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Collection:
Collection ID: | CO000349 |
Collection Summary: | On day 15 after insemination, the uterine horn ipsilateral to the corpus luteum was flushed by transcervical catheterization and infusion of 20 mL of phosphate buffered solution with 0.1% of polyvinyl acetate. The solution was infused using a 60 mL dosing plastic syringe connected in a 20 to 24 French silicone coated latex Foley catheter. The fluid infused was recovery into the same syringe used for infusion and then examined in a petri dish under stereoscope for location of conceptus tissue. The uterine fluid recovered was placed in conical tubes in ice and later centrifuged at 2,000 × g at 4ᵒC. The supernatant was collect, aliquoted and stored at -80ᵒC for later analyses of fluid composition. |
Sample Type: | Uterus |