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MB Sample ID: SA023602
Local Sample ID: | Lemos_014 |
Subject ID: | SU000487 |
Subject Type: | Bacterial cells |
Subject Species: | Streptococcus mutans |
Taxonomy ID: | 1309 |
Genotype Strain: | UA159 |
Species Group: | Microorganism |
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Collection:
Collection ID: | CO000481 |
Collection Summary: | Upon reaching OD600 = 0.4, the culture is split into 4 samples. Three are treated with H2O2 (0.5 mM). The other is harvested by filtration onto 0.45uM nitrocellulose membrane. The cells will be scraped off of the membrane into ice-cold PBS (~1 mL), centrifuged for 30 seconds at 12K RPM, liquid aspirated and stored at -80C until sending for analysis. This process will be repeated with the remaining three samples after 15, 30 and 60 minutes treatment with H2O2. |
Sample Type: | Bacterial cells |