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MB Sample ID: SA057696
| Local Sample ID: | 10_25_2016_islet_OHE_20 |
| Subject ID: | SU001003 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6N |
| Age Or Age Range: | 4 weeks old (young) or 10-15 months old (old) |
| Gender: | Male and female |
| Animal Animal Supplier: | Charles River Labs |
| Animal Feed: | standard chow diet (PicoLab Rodent Diet 20 pellets, #5053 |
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Collection:
| Collection ID: | CO000997 |
| Collection Summary: | Pancreata were digested using Liberase TL, and islets were purified using a Histopaque gradient followed by hand-picking under a dissecting microscope. Islets were allowed to recover overnight in RPMI 1640 media supplemented with 8 mM glucose, 10% FBS, 2 mM L-glutamine, 100 U/mL Pen/Strep, 1 mM sodium pyruvate, 10 mM HEPES, and 0.25 mg/mL amphoterecin B. |
| Collection Protocol Filename: | Methods.pdf |
| Sample Type: | Pancreatic Islets |
